Determination of Lymphocyte Subsets and Pcna Activity in Sheep Experimentally Infected with Bovine Leukemia Virus (blv)
نویسندگان
چکیده
Lymphocyte phenotypes were determined in sheep experimentally infected with bovine leukemia virus (BLV). BLV infection was detected by serological tests AGID and ELISA. Flow cytometry analysis with the use of specific monoclonal antibodies for CD markers and conjugates labeled with FITC or PE was performed. Proliferating cell nuclear antigen (PCNA) was estimated in lymphocytes of infected and healthy sheep by flow cytometry. Activity of acid hydrolases ANAE and AP in blood lymphocytes was also determined. The results demonstrated much higher level of PCNA in leukemic lymphocytes than in healthy ones. These values were dependent on the stage of the disease. The BLV infection caused depletion of CD4 lymphocytes in infected sheep.
منابع مشابه
FLOW CYTOMETRY DETERMINATION OF BCl-2 EXPRESSION IN ANIMALS INFECTED WITH BOVINE LEUKAEMIA VIRUS
Investigations were performed in lambs experimentally infected with blood lymphocytes of leukaemic cow. The presence of specific bovine leukaemia virus (BLV) antibodies in sera of infected animals was detected in agar gel immunodiffusion test and ELISA. Proviral DNA was detected with PCR and nested PCR. Dual-colour flow cytometry analysis with the use of specific monoclonal antibodies (for lymp...
متن کاملImmunomodulative effects of bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus on sheep.
Experimental bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus (BLV) were performed on sheep. BIV proviral DNA and anti-BIV antibodies were persistently detected in all BIV-inoculated sheep. A slight increase in lymphocyte counts was observed in BIV-infected sheep, but the percentages of CD4+ and CD8+ cells in sheep peripheral blood mononucl...
متن کاملIntestinal Shiga toxin-producing Escherichia coli bacteria mitigate bovine leukemia virus infection in experimentally infected sheep.
Ruminants often carry gastrointestinal Shiga toxin (Stx)-producing Escherichia coli (STEC). Stxs belong to a large family of ribosome-inactivating proteins (RIPs), found in many plants and some bacteria. Plant RIPs, secreted into extracellular spaces, limit the spread of viruses through plant tissues by penetrating and killing virally infected cells. Previously, we showed Stx activity against b...
متن کاملEffect of Cadmium and Lead on [h]-thymidine Uptake in Sheep Lymphocytes Infected with Bovine Leukaemia Virus
Effect of cadmium (Cd II) and lead (Pb II) was determined on cell proliferation, DNA synthesis, DNA repair, and DNA polymerase activity. The experiments were done on lymphocytes obtained from sheep infected with bovine leukaemia virus (BLV) and non-infected animals. All methods were based on [ H]-thymidine incorporation into DNA and performed in cell cultures stimulated by mitogens. Lymphocytes...
متن کاملLatency of viral expression in vivo is not related to CpG methylation in the U3 region and part of the R region of the long terminal repeat of bovine leukemia virus.
Bovine leukemia virus (BLV) is silent in most cells detectable in vivo, and the repression of its expression allows BLV to evade the host's immune response. In this study, we examined whether CpG methylation of DNA might be involved in the regulation of the expression of BLV in vivo. To investigate the effects of CpG methylation on the activity of the long terminal repeat (LTR) of BLV, we measu...
متن کامل